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Vector Red substrate kit (SK-5100) does require a Tris buffer with a minimum of 100 mM to be present for a reaction to satisfactorily occur. It is recommended to use a 200 mM Tris buffer at pH 8.2-8.5 for the most optimal staining results. If a buffer with < 100 mM Tris is used to make up the Vector Red working solution it may be that no.

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ImmPACT™ Vector® Red Substrate The ImmPACT™ Vector® Red Substrate produces a red reaction product in the presence of alkaline phosphatase (AP) enzyme. This reaction product is also highly fluores-cent and non-fading and can be viewed with Texas Red® or rhodamine filter systems.

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The bright red to orange fluorescence of the Vector Red™ reaction product can be visualized using Daylight® 594, Texas Red® or TRITC filter systems. This fluorescence is especially bright in dehydrated, permanently mounted sections. The fluorescence is stable and does not photobleach like most fluorescent dyes.

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Supplier: Vector Laboratories SK5105. ImmPACT™ Vector® Red AP Substrate Kit produces a bright red reaction product that is crisper and 2-4 times more sensitive than the original VECTOR Red Substrate. Sections stained with ImmPACT Vector Red substrate can be dehydrated, cleared, and permanently mounted. This chromogen can be used with other.

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ImmPACT® Vector® Red Alkaline Phosphatase Substrate the presence of alkaline phosphatase (AP) enzyme. This reaction product is also highly fluorescent and non-fading and can be viewed with Texas RedTM or rhodamine filter systems. Components Preparation of Substrate Working Solution To 5 ml ImmPACT Vector Red Diluent:

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Vector Red Reagent 2 4 ml Vector Red Reagent 3 4 ml Components This kit contains all the reagents necessary to prepare the substrate working solution. Vector Red Substrate produces a red reaction product in the presence of alkaline phosphatase (AP) enzyme. This reaction product is also highly fluorescent and non-fading and can be viewed with.

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The Vector® Red Substrate kit contains all of the reagents (except buffer) necessary to prepare the substrate working solution. These reagents are supplied in convenient dropper bottles. REAGENTS: STORAGE: 4 ml Reagent 1 • Store reagents in original bottles at 2-8 oC. 4 ml Reagent 2 • Avoid storing reagents or working

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Vector[R] Red AP Substrate Kit produces a magenta heat stable reaction product that is also fluorescent. Sections stained with the Vector[R] Red substrate can be dehydrated, cleared, and permanently mounted. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.

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ImmPACT Vector Red AP Substrate Kit produces a magenta reaction product that is crisper and is 2-4 times more sensitive than the original Vector ® Red substrate kit (SK-5100). It can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.